Efecto de la elicitación con metil jasmonato en la actividad antioxidante del extracto metanólico de Coleus hadiensis
Palabras clave:
Coleus hadiensis, Metil jasmonato, Elicitación, Metabolismo de las plantas
Resumen
Los metabolitos secundarios de plantas presentan actividades antioxidantes de interés farmacéutico y cosmético al neutralizar especies reactivas de oxígeno implicadas en enfermedades asociadas a daño oxidativo. La elicitación con fitohormonas como el metil jasmonato (MeJA), un potente elicitor abiótico, simula condiciones de estrés y activa rutas biosintéticas, lo que podría incrementar la cantidad de compuestos antioxidantes. Este estudio evaluó si la elicitación con MeJA aumenta la actividad antioxidante del extracto metanólico de Coleus hadiensis. La planta se elicitó durante 24 días con aplicaciones foliares dos veces por semana. Tras la elicitación se realizó un tamizaje cualitativo que confirmó fenoles, alcaloides, esteroles/esteroides y cumarinas. Se determinó el contenido fenólico total el cual fue significativamente mayor en el grupo elicitado, con un incremento aproximado de 20 mg GAE/g sobre el control. Se determinó la actividad antioxidante por DPPH, la cual aumentó de forma significativa tras la elicitación (p=0,009), consistente con una mayor capacidad captadora de radicales libres asociada al aumento de fenoles. En conjunto, la aplicación foliar de MeJA se confirma como una estrategia viable para potenciar la acumulación de metabolitos secundarios y la actividad antioxidante del extracto metanólico de C. hadiensis, incrementando su valor biotecnológico y farmacológico.
Descargas
La descarga de datos todavía no está disponible.
Citas
Adebayo E. A. Oloke J. K. Aina D. A. and Bora T. C. (2014). Antioxidant and nutritional importance of some Pleurotus species. Journal of Microbiology, Biotechnology and Food Sciences, 3(4), 289–294.
Ali, M., Prasad, G., Kumar, S., Nishad, U., & Rathaur, B. (2020). Phytochemical analysis of Tabernaemontana divaricata. Journal of Pharmacognosy and Phytochemistry, 9(2), 1283–1291.
Authinarayanan Rajesh, S. V. S. P. R. S. R. (2021). Medicinal values of a horticultural plant - Coleus hadiensis (Forssk.) A. J. Paton. International Journal of Science Letters, 3(1), 65–72. doi: 10.38058/IJSL.856837
Batool, R., Khan, M. R., Sajid, M., Ali, S., & Zahra, Z. (2019). Estimation of phytochemical constituents and in vitro antioxidant potencies of Brachychiton populneus (Schott & Endl.) R.Br. BMC Chemistry, 13(1), 1–15. doi: https://doi.org/10.1186/s13065-019-0549-z
Capecka, E., Mareczek, A., & Leja, M. (2005). Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry, 93(2), 223–226. doi: 10.1016/J.FOODCHEM.2004.09.020
Do Nascimento, L. D., de Moraes, A. A. B., da Costa, K. S., Galúcio, J. M. P., Taube, P. S., Costa, C. M. L., Cruz, J. N., Andrade, E. H. de A., & de Faria, L. J. G. (2020). Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications. Biomolecules, 10(7), 1–37. doi: 10.3390/BIOM10070988
Goossens, J., Fernández-Calvo, P., Schweizer, F., & Goossens, A. (2016). Jasmonates: signal transduction components and their roles in environmental stress responses. Plant Molecular Biology, 91(6), 673–689. doi: 10.1007/S11103-016-0480-9
Hossain, M. B., Barry-Ryan, C., Martin-Diana, A. B., & Brunton, N. P. (2010). Effect of drying method on the antioxidant capacity of six Lamiaceae herbs. Food Chemistry, 123(1), 85–91. doi: 10.1016/j.foodchem.2010.04.003
Jaqueline Moura, D., Franc xois Richter, M., Marlei Boeira, J., Antonio Pêgas Henriques, J., & Saffi, J. (2007). Antioxidant properties of b-carboline alkaloids are related to their antimutagenic and antigenotoxic activities. Mutagenesis, 22(4), 293–302. doi: 10.1093/mutage/gem016
Kim, H. J., Fonseca, J. M., Choi, J. H., & Kubota, C. (2007). Effect of Methyl Jasmonate on Phenolic Compounds and Carotenoids of Romaine Lettuce (Lactuca sativa L.). Journal of Agricultural and Food Chemistry, 55(25), 10366–10372. doi: 10.1021/JF071927M
Menon, D. B., Sasikumar, J. M., & Latha, K. (2011). Anti inflammtory and cytotoxic activity of methanolic extract of Plectranthus hadiensis stem. Pharmacologyonline, 3, 275–282. Retrieved from https://www.researchgate.net/publication/216675059
Menon D. Sasikumar J M. Latha K. (2012). Phytochemical analysis and antioxidant activity of methanolic extract of Plectranthus hadiensis (Forssk.) Schweinf. ex Spreng. aerial parts. Indian Journal of Natural Products and Resources, 3(3), 359–365.
Mirjana Marčetić, J. A. (2023). Antioxidant activity of plant secondary metabolites. Arhiv Za Farmaciju, 73, 264–277. doi: doi.org/10.5937/arhfarm73-45560
Namdeo A.G. (2007). Plant Cell Elicitation for Production of Secondary Metabolites: A Review. Pharmacognosy Reviews, 1(1), 69–79.
Phuyal, N., Jha, P. K., Raturi, P. P., & Rajbhandary, S. (2020). Total Phenolic, Flavonoid Contents, and Antioxidant Activities of Fruit, Seed, and Bark Extracts of Zanthoxylum armatum DC. The Scientific World Journal, 2020, 1–7. doi: https://doi.org/10.1155/2020/8780704
Rahdari, F. G., Shahanipour, K., Monajemi, R., & Adibnejad, M. (2022). Antioxidant and cytotoxic potentials of the methanolic extract of Teucrium persicum Boiss. in A-375 melanoma cells. Avicenna Journal of Phytomedicine, 12(2), 175–184. doi: 10.22038/AJP.2021.19308
Rahimmalek, M., Afshari, M., Sarfaraz, D., & Miroliaei, M. (2020). Using HPLC and multivariate analyses to investigate variations in the polyphenolic compounds as well as antioxidant and antiglycative activities of some Lamiaceae species native to Iran. Industrial Crops and Products, 154, 1–9. doi: 10.1016/J.INDCROP.2020.112640
Resmi Mustarichie, D. R. (2021). ANTIOXIDANT OF COLEUS SCUTELLARIOIDES L. WATER EXTRACTS BY DPPH METHOD. Journal of Bio Innovation, 10(6), 1725–1732. doi: https://doi.org/10.46344/JBINO.2021.v10i06.23
Safeena, M., & Kalinga, J. (2020). Qualitative and quantitative screening of secondary metabolites in selected medicinal plants of Sri Lanka. Journal of Medicinal Plants Studies, 8(5), 94–100.
Saggar, S., Mir, P. A., Kumar, N., Chawla, A., Uppal, J., Shilpa, S., & Kaur, A. (2022). Traditional and Herbal Medicines: Opportunities and Challenges. Pharmacognosy Research, 14(2), 107–114. doi: 10.5530/PRES.14.2.15
Salazar, J. R., Loza-Mejía, M. A., Soto-Cabrera, D., Rastelli, G., Pellati, F., & Pinzi, L. (2020). Chemistry, Biological Activities and In Silico Bioprospection of Sterols and Triterpenes from Mexican Columnar Cactaceae. Molecules, 25(7), 1–34. doi: 10.3390/MOLECULES25071649
Shahzad, N., Khan, W., MD, S., Ali, A., Saluja, S. S., Sharma, S., Al-Allaf, F. A., Abduljaleel, Z., Ibrahim, I. A. A., Abdel-Wahab, A. F., Afify, M. A., & Al-Ghamdi, S. S. (2017). Phytosterols as a natural anticancer agent: Current status and future perspective. Biomedicine & Pharmacotherapy, 88, 786–794. doi: 10.1016/J.BIOPHA.2017.01.068
Stefanachi, A., Leonetti, F., Pisani, L., Catto, M., & Carotti, A. (2018). Coumarin: A Natural, Privileged and Versatile Scaffold for Bioactive Compounds. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(2), 1–34. doi: 10.3390/MOLECULES23020250
Talebi, M., Moghaddam, M., & Pirbalouti, A. G. (2018). Methyl jasmonate effects on volatile oil compounds and antioxidant activity of leaf extract of two basil cultivars under salinity stress. Acta Physiologiae Plantarum, 40(2), 1–11. doi: 10.1007/S11738-018-2611-1
Thangaraj, P. (2016). Quantification of Secondary Metabolites. Progress in Drug Research, 71, 49–55. doi: 10.1007/978-3-319-26811-8_8
Uzma Salam. Shakir Ullah. Zhong-Hua Tang. Ahmed A. Elateeq, Yaseen Khan. Jafar Khan. Asif Khan and Sajid Ali. (2023). Plant Metabolomics: An Overview of the Role of Primary and Secondary Metabolites against Different Environmental Stress Factors. Life, 13(3), 1–25. doi: 10.3390/LIFE13030706
Wazir, M. A., Azhar, M. I., Mehmood, Z. A., Qadir, M. I., Khan, M. Y., Siddique, F. A., Shaheer, T., Hussain, W., & Abbas, K. (2020). Pharmacognostic, phytochemical, biological and spectroscopic analyses of Capparis decidua (Forsk.) Edgew root and stem bark. Tropical Journal of Pharmaceutical Research, 19(12), 2667–2675. doi: 10.4314/tjpr.v19i12.27
Yousefian, S., Lohrasebi, T., Farhadpour, M., & Haghbeen, K. (2020). Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis-related genes in Mentha spicata hairy root cultures. Plant Cell, Tissue and Organ Culture, 142(2), 285–297. doi: 10.1007/S11240-020-01856-9
Zhou, W., Shi, M., Deng, C., Lu, S., Huang, F., Wang, Y., & Kai, G. (2021). The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza. Horticulture Research, 8(1), 1–13. doi: 10.1038/S41438-020-00443-5
Ali, M., Prasad, G., Kumar, S., Nishad, U., & Rathaur, B. (2020). Phytochemical analysis of Tabernaemontana divaricata. Journal of Pharmacognosy and Phytochemistry, 9(2), 1283–1291.
Authinarayanan Rajesh, S. V. S. P. R. S. R. (2021). Medicinal values of a horticultural plant - Coleus hadiensis (Forssk.) A. J. Paton. International Journal of Science Letters, 3(1), 65–72. doi: 10.38058/IJSL.856837
Batool, R., Khan, M. R., Sajid, M., Ali, S., & Zahra, Z. (2019). Estimation of phytochemical constituents and in vitro antioxidant potencies of Brachychiton populneus (Schott & Endl.) R.Br. BMC Chemistry, 13(1), 1–15. doi: https://doi.org/10.1186/s13065-019-0549-z
Capecka, E., Mareczek, A., & Leja, M. (2005). Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry, 93(2), 223–226. doi: 10.1016/J.FOODCHEM.2004.09.020
Do Nascimento, L. D., de Moraes, A. A. B., da Costa, K. S., Galúcio, J. M. P., Taube, P. S., Costa, C. M. L., Cruz, J. N., Andrade, E. H. de A., & de Faria, L. J. G. (2020). Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications. Biomolecules, 10(7), 1–37. doi: 10.3390/BIOM10070988
Goossens, J., Fernández-Calvo, P., Schweizer, F., & Goossens, A. (2016). Jasmonates: signal transduction components and their roles in environmental stress responses. Plant Molecular Biology, 91(6), 673–689. doi: 10.1007/S11103-016-0480-9
Hossain, M. B., Barry-Ryan, C., Martin-Diana, A. B., & Brunton, N. P. (2010). Effect of drying method on the antioxidant capacity of six Lamiaceae herbs. Food Chemistry, 123(1), 85–91. doi: 10.1016/j.foodchem.2010.04.003
Jaqueline Moura, D., Franc xois Richter, M., Marlei Boeira, J., Antonio Pêgas Henriques, J., & Saffi, J. (2007). Antioxidant properties of b-carboline alkaloids are related to their antimutagenic and antigenotoxic activities. Mutagenesis, 22(4), 293–302. doi: 10.1093/mutage/gem016
Kim, H. J., Fonseca, J. M., Choi, J. H., & Kubota, C. (2007). Effect of Methyl Jasmonate on Phenolic Compounds and Carotenoids of Romaine Lettuce (Lactuca sativa L.). Journal of Agricultural and Food Chemistry, 55(25), 10366–10372. doi: 10.1021/JF071927M
Menon, D. B., Sasikumar, J. M., & Latha, K. (2011). Anti inflammtory and cytotoxic activity of methanolic extract of Plectranthus hadiensis stem. Pharmacologyonline, 3, 275–282. Retrieved from https://www.researchgate.net/publication/216675059
Menon D. Sasikumar J M. Latha K. (2012). Phytochemical analysis and antioxidant activity of methanolic extract of Plectranthus hadiensis (Forssk.) Schweinf. ex Spreng. aerial parts. Indian Journal of Natural Products and Resources, 3(3), 359–365.
Mirjana Marčetić, J. A. (2023). Antioxidant activity of plant secondary metabolites. Arhiv Za Farmaciju, 73, 264–277. doi: doi.org/10.5937/arhfarm73-45560
Namdeo A.G. (2007). Plant Cell Elicitation for Production of Secondary Metabolites: A Review. Pharmacognosy Reviews, 1(1), 69–79.
Phuyal, N., Jha, P. K., Raturi, P. P., & Rajbhandary, S. (2020). Total Phenolic, Flavonoid Contents, and Antioxidant Activities of Fruit, Seed, and Bark Extracts of Zanthoxylum armatum DC. The Scientific World Journal, 2020, 1–7. doi: https://doi.org/10.1155/2020/8780704
Rahdari, F. G., Shahanipour, K., Monajemi, R., & Adibnejad, M. (2022). Antioxidant and cytotoxic potentials of the methanolic extract of Teucrium persicum Boiss. in A-375 melanoma cells. Avicenna Journal of Phytomedicine, 12(2), 175–184. doi: 10.22038/AJP.2021.19308
Rahimmalek, M., Afshari, M., Sarfaraz, D., & Miroliaei, M. (2020). Using HPLC and multivariate analyses to investigate variations in the polyphenolic compounds as well as antioxidant and antiglycative activities of some Lamiaceae species native to Iran. Industrial Crops and Products, 154, 1–9. doi: 10.1016/J.INDCROP.2020.112640
Resmi Mustarichie, D. R. (2021). ANTIOXIDANT OF COLEUS SCUTELLARIOIDES L. WATER EXTRACTS BY DPPH METHOD. Journal of Bio Innovation, 10(6), 1725–1732. doi: https://doi.org/10.46344/JBINO.2021.v10i06.23
Safeena, M., & Kalinga, J. (2020). Qualitative and quantitative screening of secondary metabolites in selected medicinal plants of Sri Lanka. Journal of Medicinal Plants Studies, 8(5), 94–100.
Saggar, S., Mir, P. A., Kumar, N., Chawla, A., Uppal, J., Shilpa, S., & Kaur, A. (2022). Traditional and Herbal Medicines: Opportunities and Challenges. Pharmacognosy Research, 14(2), 107–114. doi: 10.5530/PRES.14.2.15
Salazar, J. R., Loza-Mejía, M. A., Soto-Cabrera, D., Rastelli, G., Pellati, F., & Pinzi, L. (2020). Chemistry, Biological Activities and In Silico Bioprospection of Sterols and Triterpenes from Mexican Columnar Cactaceae. Molecules, 25(7), 1–34. doi: 10.3390/MOLECULES25071649
Shahzad, N., Khan, W., MD, S., Ali, A., Saluja, S. S., Sharma, S., Al-Allaf, F. A., Abduljaleel, Z., Ibrahim, I. A. A., Abdel-Wahab, A. F., Afify, M. A., & Al-Ghamdi, S. S. (2017). Phytosterols as a natural anticancer agent: Current status and future perspective. Biomedicine & Pharmacotherapy, 88, 786–794. doi: 10.1016/J.BIOPHA.2017.01.068
Stefanachi, A., Leonetti, F., Pisani, L., Catto, M., & Carotti, A. (2018). Coumarin: A Natural, Privileged and Versatile Scaffold for Bioactive Compounds. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(2), 1–34. doi: 10.3390/MOLECULES23020250
Talebi, M., Moghaddam, M., & Pirbalouti, A. G. (2018). Methyl jasmonate effects on volatile oil compounds and antioxidant activity of leaf extract of two basil cultivars under salinity stress. Acta Physiologiae Plantarum, 40(2), 1–11. doi: 10.1007/S11738-018-2611-1
Thangaraj, P. (2016). Quantification of Secondary Metabolites. Progress in Drug Research, 71, 49–55. doi: 10.1007/978-3-319-26811-8_8
Uzma Salam. Shakir Ullah. Zhong-Hua Tang. Ahmed A. Elateeq, Yaseen Khan. Jafar Khan. Asif Khan and Sajid Ali. (2023). Plant Metabolomics: An Overview of the Role of Primary and Secondary Metabolites against Different Environmental Stress Factors. Life, 13(3), 1–25. doi: 10.3390/LIFE13030706
Wazir, M. A., Azhar, M. I., Mehmood, Z. A., Qadir, M. I., Khan, M. Y., Siddique, F. A., Shaheer, T., Hussain, W., & Abbas, K. (2020). Pharmacognostic, phytochemical, biological and spectroscopic analyses of Capparis decidua (Forsk.) Edgew root and stem bark. Tropical Journal of Pharmaceutical Research, 19(12), 2667–2675. doi: 10.4314/tjpr.v19i12.27
Yousefian, S., Lohrasebi, T., Farhadpour, M., & Haghbeen, K. (2020). Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis-related genes in Mentha spicata hairy root cultures. Plant Cell, Tissue and Organ Culture, 142(2), 285–297. doi: 10.1007/S11240-020-01856-9
Zhou, W., Shi, M., Deng, C., Lu, S., Huang, F., Wang, Y., & Kai, G. (2021). The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza. Horticulture Research, 8(1), 1–13. doi: 10.1038/S41438-020-00443-5
Publicado
2026-07-16
Cómo citar
Valdez-Arellanes, A., Recendiz-Cerda, J., Torres-Contreras, A., & Ramírez-Estrada, K. (2026). Efecto de la elicitación con metil jasmonato en la actividad antioxidante del extracto metanólico de Coleus hadiensis, 8(15), 52-66. https://doi.org/10.5281/zenodo.21386365
Número
Sección
Ciencias Naturales, Exactas, del Agro, de la Salud e Ingeniería
Derechos de autor 2026 Ana Laura Valdez-Arellanes, José Abraham Recendiz-Cerda, Ana Mariel Torres-Contreras, Karla Ramírez-Estrada
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
.png)
.png){kind=logo}
